Al-Hikmah University Journal

Objective: This study investigated the probable haemopoietic multi-stem cells differentiation enhancing effects of Parquetina nigrescens, Camellia sinensis and Telfairia occidentalis leaves extracts. Materials and Methods: Haemopoietic bone marrow stem cells from irradiated guinea pigs were harvested on day 13th post-irradiation, cultured and treated with varying concentrations (0.313-100%) of aqueous leaf extracts of each of the plant extracts. The degree of differentiation was determined by MTT assay. Results: The degree of differentiation was highest in Telfairia occidentalis, followed by Camellia sinensis and Parquetina nigrescens respectively. Combination of the three plants showed a synergistic stimulatory effect on cell differentiation. Also, strong positive correlation between the concentrations of each of the extract of the three plants and the degree of differentiation. Conclusion: Camellia sinensis, Telfairia occidentalis and Parquetina nigrescens leaves extracts have positive differentiation enhancing effects on haemopoietic multi-potent stem cells individually and when combined.